Response: High-Affinity Potassium Uptake in Plants

High-affinity potassium uptake in plants.

oocyte]. Oocytes were incubated for 24 to 48 hours, treated with coelenterazine [10 ,uM coelenterazine (Molecular Probes, Eugene, OR) and 30 p.M reduced glutathione in OR-2 media (no calcium)] for 2 to 3 hours with gentle orbital shaking in the dark at 18°C, and retumed to ND-86 medium with calcium (maintained in the dark with shaking) until measurements were initiated. For luminometer measurem...

High-Affinity Potassium Uptake Transporter from Higher Plants

High-affinity sodium uptake in land plants.

High-affinity Na(+) uptake in plants and its mediation by HKT transporters have been studied in very few species. This study expands the knowledge of high-affinity Na(+) uptake in land plants for both uptake characteristics and involvement of HKT transporters. In non-flowering plants, we analyzed the Na(+) content of wild mosses, carried out experiments on K(+) and Na(+) uptake in the micromola...

High-affinity potassium and sodium transport systems in plants.

All living cells have an absolute requirement for K+, which must be taken up from the external medium. In contrast to marine organisms, which live in a medium with an inexhaustible supply of K+, terrestrial life evolved in oligotrophic environments where the low supply of K+ limited the growth of colonizing plants. In these limiting conditions Na+ could substitute for K+ in some cellular functi...

Low-affinity potassium uptake system in Bacillus acidocaldarius.

Cells of Bacillus acidocaldarius that were grown with 2.7 mM K+ expressed a low-affinity K+ uptake system. The following observations indicate that its properties closely resemble those of the Escherichia coli Trk and Streptococcus faecalis KtrI systems: (i) the B. acidocaldarius system took up K+ with a Km of 1 mM; (ii) it accepted Rb+ (Km of 6 mM; same Vmax as for K+); (iii) it was still acti...